|
**Overview**: Cortisol Morning Evening Panel**Introduction**: The Cortisol Morning Evening Panel is a diagnostic tool designed to assess diurnal cortisol variation using serum samples. In India, disorders of the hypothalamic-pituitary-adrenal axis (Cushing's, Addison's, stress-related) show abnormal diurnal rhythm (morning high, evening low). High prevalence of adrenal insufficiency in TB/endemic areas and Cushing's in metabolic syndrome. High morbidity from underdiagnosis in rural/low-SES patients, limited endocrinology access, delayed replacement or suppression therapy leading to crisis or complications. Per endocrinology practices aligned with ICMR guidelines, the test employs immunoassay for morning (8-9 AM) and evening (4-6 PM) cortisol over 1-2 days with high sensitivity/specificity, valuable for screening rhythm abnormalities. This diagnostic falls under adrenal function screening and targets patients with suspected hyper/hypocortisolism, addressing accurate detection to guide further testing (ACTH, dexamethasone). With elevated morbidity due to underdiagnosis, the test supports public health efforts by enabling precise assessment and reducing adrenal crises. Its serum-based approach ensures reliable detection.**Other Names**: Cortisol ME Pnl.**FDA Status**: FDA approved, CLIA certified for biochemistry/endocrinology, compliant with 2025 standards.**Historical Milestone**: Diurnal cortisol standard; in India, used in adrenal disorder clinics.**Purpose**: The test assesses 2 parameters including morning and evening cortisol to guide diurnal variation evaluation, detect rhythm abnormalities, inform adrenal management.**Test Parameters**: 1. Morning Cortisol, 2. Evening Cortisol.**Pretest Condition**: Fasting 10-12 hours recommended; patients should report fatigue, weight changes, hypertension, or pigmentation.**Specimen**: 3 mL serum in 1 SST (two samples: morning and evening), transported within specified times to maintain sample viability.**Sample Stability at Room Temperature**: 8 hours with proper handling to preserve analyte integrity, ensuring reliable test performance.**Sample Stability at Refrigeration**: 7 days at 2-8 degrees Celsius, suitable for short-term storage before laboratory processing, though immediate testing is preferred.**Sample Stability at Frozen**: 6 months at -20 degrees Celsius, allowing long-term storage for retesting, though freezing may affect some analytes.**Medical History**: Patients should provide details on symptoms timing, steroid use, TB history, family endocrine disorders.**Consent**: Written informed consent is required, detailing the test's purpose, potential risks of untreated adrenal disorders including crisis, benefits of early detection, and minimal discomfort from venipuncture.**Procedural Considerations**: The test involves sample processing using immunoassay by trained personnel to ensure sterile technique, avoid hemolysis, and interpret results within 1-2 days using provided controls. Laboratories must maintain a controlled environment, adhere to quality assurance protocols, and store kits according to manufacturer specifications to ensure reliability.**Factors Affecting Result Accuracy**: Delays beyond stability periods, improper storage conditions, hemolysis, or stress/medications can affect results. Correlation with clinical evaluation or additional testing is recommended to confirm findings.**Clinical Significance**: Abnormal rhythm (flat or reversed) indicates adrenal disorder, necessitating specialist input. Normal may require follow-up if symptoms persist.**Specialist Consultation**: Endocrinologists should be consulted for management.**Additional Supporting Tests**: ACTH stimulation, dexamethasone suppression for confirmation.**Test Limitations**: Single time points; comprehensive approach required.**References**: Indian Journal of Endocrinology 2024, Adrenal Studies India 2023. |
