Overview: Soluble Transferrin Receptor TestIntroduction: The Soluble Transferrin Receptor Test measures sTfR to diagnose iron deficiency anemia, causing fatigue or pallor. Following 2023 ASH guidelines, it uses biochemical methods for high specificity, supporting blood disorder screening. This test is critical for guiding diagnosis, treatment planning, and improving outcomes in biochemistry for patients with suspected iron deficiency.
Other Names: sTfR Assay, Transferrin Receptor Test.
FDA Status: Laboratory-developed test (LDT), meeting biochemistry standards for diagnostic accuracy.
Historical Milestone: sTfR testing began in the 1980s with anemia research. Biochemical methods improved in the 2000s, enhancing diagnostic precision.
Purpose: Measures sTfR to diagnose iron deficiency anemia, guides treatment, and evaluates patients with fatigue or pallor.
Test Parameters: 1. sTfR Level
Pretest Condition: No fasting required. Collect serum or plasma. Report history of fatigue, pallor, or anemia symptoms.
Specimen: Serum (SST, 2-5 mL), Plasma (EDTA, 2-4 mL). Transport in a biohazard container.
Sample Stability at Room Temperature: 8 hours
Sample Stability at Refrigeration: 7 days
Sample Stability at Frozen: 6 months
Medical History: Document fatigue, pallor, or history of anemia. Include current medications, especially iron supplements.
Consent: Written consent required, detailing the tests purpose, iron deficiency anemia implications, and risks of sample collection.
Procedural Considerations: Uses enzyme-linked immunosorbent assay (ELISA) to measure sTfR levels. Results are available in 1-2 days, supporting clinical decisions. Performed in laboratories, often for iron deficiency diagnosis.
Factors Affecting Result Accuracy: Sample hemolysis or improper storage can affect results. Inflammation may elevate sTfR levels.
Clinical Significance: Elevated sTfR confirms iron deficiency anemia, guiding iron supplementation. Normal levels may require ferritin or TIBC testing.
Specialist Consultation: Consult a hematologist for result interpretation and treatment planning.
Additional Supporting Tests: Ferritin, TIBC, or CBC to confirm iron deficiency anemia diagnosis.
Test Limitations: Non-specific in the presence of inflammation; clinical correlation is needed. Sample quality affects sensitivity.
References: ASH Anemia Guidelines, 2023; Blood, Cook JD, 2022.
